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Goodbye Ethidium Bromide!
Biomoles’ new generation RedTrack™ and GreenTrack™ Precast Gels use newly developed staining substances to provide superior imaging without touching the mutagen ethidium bromide or unstable SYBR green. The resulting images are remarkably sharp and clear bands; responsive to minimal amount of DNA; uncover small fragments; and very clean background. The products are safer for the scientists and easier to handle. RedTrack Gels can be used with any standard UV transilluminator. GreenTrack Gels can be used with SYBR reader, short wave UV transiluminator and laser based gel reader.
Introduction
Both Gels are delivered in our UV transparent SureTrack tray. The SureTrack tray has specially designed walls that protect the gels during transport and storage but can be easily removed to provide direct gel-to-buffer contact in any standard electrophoresis device. The ultra-clear and UV transparent trays allow for convenient repeating observations of DNA/RNA mobility and high quality imaging without ever having to touch the gel. The Gels can remain in the tray throughout the entire electrophoresis process. The results are faster analysis times, consistent results, and optimal imaging.
Competitive Advantages:
- Safer to Use
- Does not contain Ethidium Bromide.
- According to Ames test, RedTrack™ and GreenTrack™ stains showed undetectable muta-genicity and toxicity with the concentration used in RedTrack™ & GreenTrack™ Precast Gels.
- Four sided tray keeps everything clean.
- Yields High Quality Results
- Significantly sharper bands than using ethidium bromide.
- Very clean background.
- Ultra-clear tray material.
- High resolution DNA/RNA bands.
- Highly Sensitive
- About 10-50 times higher sensitivity to DNA/RNA.
- Requires minimal amount DNA: as little as 1.7ng per lane for genome DNA and 4.0ng for 10bp.
- Easy, Convenient, Saves Time
- Easy to plan your experiments.
- Easy observation and imaging.
- High level of consistency.
- Simple access for DNA recovery.
- DNA/RNA bands can be observed within 15 minutes.
- Provides Consistent Results.
- Undetectable DNase and RNase.
- Consistent gel concentration.
- Eliminate gel handling related contamination and damage.
- Avoids problems with SYBR green temperature stability.
- Universal Application
- No special equipment required, fits into standard electrophoresis chambers.
- Available in a variety of well formats and agarose concentrations.
- No need to change the filters: RedTrack™ Precast Gels can be analyzed under standard UV transiluminator or SYBR reader; GreenTrack™ Precast gels can be analyzed under SYBR reader, 254nm UV transiluminator or 488nm laser based gel reader.
Electrophoresis Reference Table
| Agarose Concentration |
Recommended Fragment Size |
Functional Size Range |
Gel Running Time |
| 0.8% |
4,000-15,000bp |
300-15,000bp |
20-45 min. |
| 1.0% |
3,500-5,000bp |
100-10,000bp |
15-30 min. |
| 1.5% |
2,000-3,500bp |
70-10,000bp |
15-25 min. |
| 2.0% |
1,000-2,000bp |
50-4,000bp |
15-25 min. |
| 3.0% |
500-1,000bp |
50-1,500bp |
15-30 min. |
| 4.0% |
10-500bp |
10-1000bp |
10-25 min. |
Catalog for RedTrack™ & GreenTrack™ Precast Agarose Gels
| Concentration |
Well Format |
Gel Dimensions |
Product No. |
Additional
Coding |
|
| % Agarose |
No. Wells |
No. Row x
Wells/Row |
LxW |
Catalog No. |
Buffer |
Coding |
| 0.8% |
8 |
1x8 |
10x7 |
08-08-107 |
RedTrack |
RT |
| 0.8% |
14 |
1x14 |
6x10 |
08-14-610 |
GreenTrack |
GT |
| 0.8% |
16 |
2x8 |
10x7 |
08-16-107 |
|
|
| 1.0% |
8 |
1x8 |
10x7 |
10-08-107 |
|
|
| 1.0% |
14 |
1x14 |
6x10 |
10-14-610 |
|
|
| 1.0% |
16 |
2x8 |
10x7 |
10-16-107 |
Sizes |
|
| 1.5% |
8 |
1x8 |
10x7 |
15-08-107 |
20 Units |
20 |
| 1.5% |
14 |
1x14 |
6x10 |
15-14-610 |
30 Units |
30 |
| 1.5% |
16 |
2x8 |
10x7 |
15-16-107 |
|
|
| 2.0% |
8 |
1x8 |
10x7 |
20-08-107 |
|
|
| 2.0% |
14 |
1x14 |
6x10 |
20-14-610 |
|
|
| 2.0% |
16 |
2x8 |
10x7 |
20-16-107 |
|
|
| 3.0% |
8 |
1x8 |
10x7 |
30-08-107 |
|
|
| 3.0% |
14 |
1x14 |
6x10 |
30-14-610 |
|
|
| 3.0% |
16 |
2x8 |
10x7 |
30-16-107 |
|
|
| 4.0% |
8 |
1x8 |
10x7 |
40-08-107 |
|
|
| 4.0% |
14 |
1x14 |
6x10 |
40-14-610 |
|
|
| 4.0% |
16 |
2x8 |
10x7 |
40-16-107 |
|
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Buffers and Loading Dye
| Catalog
No. |
Product |
Unit Size |
Concentration |
| BTA-10M1 |
10xTAE Buffer |
1000ml |
10 |
| BTA-50M1 |
50xTAE Buffer |
1000ml |
50 |
| BTB-10M1 |
10xTBE Buffer |
1000ml |
10 |
| BTE-05M1 |
5xTBE Buffer |
1000ml |
5 |
| RDNA-6M1 |
Red/Orange DNA Loading Dye; Red, Green Tracks |
1ml |
6x |
| RDNA-6M5 |
Red/Orange DNA Loading Dye; Red, Green Tracks |
5ml |
6x |
To Order: Contact Us.
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